nonlinear curve fitting with dose–response function and levenberg marquardt iteration algorithm Search Results


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levenberg–marquardt algorithm  (GraphPad Software Inc)
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levenberg– marquardt algorithm  (OriginLab corp)
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nonlinear curve fitting with dose–response function and levenberg marquardt iteration algorithm  (OriginLab corp)
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In vitro inhibition by calotropin and ouabain of nine α/β-complexes of O. fasciatus expressed heterologously in cell culture. Dose response relationships between increasing cardenolide concentrations and residual enzyme activities were expressed in % of the non-inhibited control (calculated with Origin Pro 9.1G). Each data point represents the mean of three biological replicates and standard deviation. Effect of calotropin (blue curves and round symbols) and ouabain (black curves and square symbols) a) on αA, b) on αB, and c) on αC combined each with β1, 2, and 3. Please note the different scales.
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In vitro inhibition by calotropin and ouabain of nine α/β-complexes of O. fasciatus expressed heterologously in cell culture. Dose response relationships between increasing cardenolide concentrations and residual enzyme activities were expressed in % of the non-inhibited control (calculated with Origin Pro 9.1G). Each data point represents the mean of three biological replicates and standard deviation. Effect of calotropin (blue curves and round symbols) and ouabain (black curves and square symbols) a) on αA, b) on αB, and c) on αC combined each with β1, 2, and 3. Please note the different scales.

Journal: bioRxiv

Article Title: Different combinations of insect Na,K-ATPase α- and β-subunit paralogs enable fine tuning of toxin resistance and enzyme kinetics

doi: 10.1101/2020.08.28.272054

Figure Lengend Snippet: In vitro inhibition by calotropin and ouabain of nine α/β-complexes of O. fasciatus expressed heterologously in cell culture. Dose response relationships between increasing cardenolide concentrations and residual enzyme activities were expressed in % of the non-inhibited control (calculated with Origin Pro 9.1G). Each data point represents the mean of three biological replicates and standard deviation. Effect of calotropin (blue curves and round symbols) and ouabain (black curves and square symbols) a) on αA, b) on αB, and c) on αC combined each with β1, 2, and 3. Please note the different scales.

Article Snippet: The data of each Na,K-ATPase α/β-subunit construct was subjected to nonlinear curve fitting with dose response function and Levenberg Marquardt iteration algorithm in Origin-Pro 9.1G (OriginLab Corporation, Northampton, USA) using a four parameter logistic function, top asymptote was set to 100, bottom asymptote set to 0 and a variable Hill slope.

Techniques: In Vitro, Inhibition, Cell Culture, Standard Deviation